{"id":619,"date":"2019-01-27T10:51:06","date_gmt":"2019-01-27T10:51:06","guid":{"rendered":"http:\/\/clinicalbiochemistry.net\/?page_id=619"},"modified":"2019-01-27T10:59:23","modified_gmt":"2019-01-27T10:59:23","slug":"mscs-loaded-with-p5","status":"publish","type":"page","link":"http:\/\/clinicalbiochemistry.net\/?page_id=619","title":{"rendered":"MSCs loaded with p5"},"content":{"rendered":"\n

Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells<\/strong><\/p>\n\n\n\n

Wen-Hui Fang, Shant Kumar, Garry McDowell, David Smith, Jurek Krupinski, Peter Olah, Raid Saleem Al-Baradie, Mohammad Othman Al-Rukban, Eugene Bogdan Petcu, and Mark Slevin.<\/p>\n\n\n\n

The potential use of stem cells as therapeutics in disease has gained momentum over the last few years and recently phase-I clinical trials have shown favourable results in treatment of a small cohort of acute stroke patients. Similarly, they have been used in preclinical models drug-loaded for the effective treatment of solid tumours. Here we have characterized uptake and release of a novel p5-cyclin-dependent kinase 5 (CDK5) inhibitory peptide by mesenchymal stem cells and showed release levels capable of blocking aberrant cyclin-dependent kinase 5 (CDK5) signaling pathways, through phosphorylation of cyclin-dependent kinase 5 (CDK5) and p53. These pathways represent the major acute mechanism stimulating apoptosis after stroke and hence its modulation could benefit patient recovery. This work indicates a potential use for drug-loaded stem cells as delivery vehicles for stroke therapeutics and in addition as anticancer receptacles particularly, if a targeting and\/or holding mechanism can be defined.<\/p>\n\n\nFull Text<\/span><\/a>","protected":false},"excerpt":{"rendered":"

Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells Wen-Hui Fang, Shant Kumar, Garry McDowell, David Smith, Jurek Krupinski, Peter Olah, Raid Saleem Al-Baradie, Mohammad Othman Al-Rukban, Eugene Bogdan Petcu, and<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"_links_to":"","_links_to_target":""},"jetpack_shortlink":"https:\/\/wp.me\/P9tPlw-9Z","jetpack-related-posts":[{"id":121,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=121","url_meta":{"origin":619,"position":0},"title":"Research Papers","date":"December 22, 2017","format":false,"excerpt":"Body, R., McDowell, G., Carley, S., Ferguson, J. & Mackway-Jones, K. (2010) \u2018Diagnosing acute myocardial infarction with troponins: how low can you go?\u2019 Emerg Med J. 27 pp. 292-296. Body, R., Carley, S., Wibberley, C., McDowell, G., Ferguson, J. & Mackway- Jones, K. (2010) \u2018The value of symptoms and signs\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":499,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=499","url_meta":{"origin":619,"position":1},"title":"Acetlycholine & Monomeric C-Reactive Protein","date":"January 1, 2019","format":false,"excerpt":"Slevin, M., Lemma, R.S., Zeinolabediny, Y., Liu, D., Ferris, G.R., Caprio, V., Phillips, N., Di Napoli, M., Guo, B., Zeng, X., AlBaradie, R., Binsaleh, N.K.,\u00a0McDowell, G. & Fang, W-H. (2018) Acetylcholine inhibits monomeric C-Reactive Protein inducd inflammation, endothelial cell adhesion and platelet aggregation; A potential therapeutic. Frontiers in Immunology 9\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"https:\/\/i0.wp.com\/clinicalbiochemistry.net\/wp-content\/uploads\/2017\/12\/Lab-Equipment-Pages.png?fit=800%2C534&resize=350%2C200","width":350,"height":200},"classes":[]},{"id":193,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=193","url_meta":{"origin":619,"position":2},"title":"Book Chapter: Thyroid Function","date":"December 24, 2017","format":false,"excerpt":"McDowell, G. (2016) \u2018Thyroid Disease\u2019. In: Admed, N. (ed) (2016) Fundamentals of Biomedical Science Clinical Biochemistry. 2nd Edition. Oxford University Press. pp 284-299. (16 pages) \u00a0 Learning objectives After studying this chapter you should be able to: Describe the structure and function of the thyroid gland Explain the function of\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"https:\/\/i0.wp.com\/clinicalbiochemistry.net\/wp-content\/uploads\/2017\/12\/Clinical-Biochemistry-Book-pages-1-227x300.png?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":850,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=850","url_meta":{"origin":619,"position":3},"title":"Confounding patient and treatment parameters on morphokinetics","date":"February 20, 2021","format":false,"excerpt":"An investigation into the effect of potential confounding patient and treatment parameters on an embryo\u2019s morphokinetic profile Objective: To determine the effect of patient and treatment parameters on nineteen embryo morphokinetic parameters using pronuclear fading as time-zero.\u00a0 Patients: Patients undergoing treatment between September 2014 and January 2016 (n=639) whose embryos\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":191,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=191","url_meta":{"origin":619,"position":4},"title":"Book Chapter: Abnormal Pituitary Function","date":"December 24, 2017","format":false,"excerpt":"McDowell, G. (2016) \u2018Abnormal Pituitary Function\u2019. In: Admed, N. (ed) (2016) Fundamentals of Biomedical Science Clinical Biochemistry. 2nd Edition Oxford University Press. pp 263-283. (21 pages) \u00a0 Learning objectives After studying this chapter you should be able to: Describe the basic structure and function of the endocrine system Explain the\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"https:\/\/i0.wp.com\/clinicalbiochemistry.net\/wp-content\/uploads\/2017\/12\/Clinical-Biochemistry-Book-pages-227x300.png?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":261,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=261","url_meta":{"origin":619,"position":5},"title":"Time lapse embryo culture","date":"December 31, 2017","format":false,"excerpt":"Barrie, A., Homberg, R., McDowell, G., Brown, J., Kingsland, C., Troup, S. (2017) Embryos cultured in a time lapse system result in superior treatment outcomes: A strict matched pair analysis. Human Fertility 20 pp. 179-185. A retrospective, strict matched-pair analysis on 728 treatment cycles between January 2011 and September 2014\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"_links":{"self":[{"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/pages\/619"}],"collection":[{"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=619"}],"version-history":[{"count":2,"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/pages\/619\/revisions"}],"predecessor-version":[{"id":623,"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=\/wp\/v2\/pages\/619\/revisions\/623"}],"wp:attachment":[{"href":"http:\/\/clinicalbiochemistry.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}