{"id":259,"date":"2017-12-31T09:55:41","date_gmt":"2017-12-31T09:55:41","guid":{"rendered":"http:\/\/clinicalbiochemistry.net\/?page_id=259"},"modified":"2019-01-19T09:25:39","modified_gmt":"2019-01-19T09:25:39","slug":"inter-observer-variability-of-h-fabp","status":"publish","type":"page","link":"http:\/\/clinicalbiochemistry.net\/?page_id=259","title":{"rendered":"Inter-Observer Variability of H-FABP"},"content":{"rendered":"

Mashali, M., Norris, N., McDowell, G<\/strong>. & Body, R. (2016) The inter-observer reliability of a novel qualitative point of care assay for heart-type fatty acid binding protein.<\/strong> Clin Biochem 49 pp. 1199-1201<\/p>\n

\u00a0<\/p>\n

Background<\/em><\/strong><\/p>\n

\"\"Heart-type fatty acid\u2013binding protein (h-FABP) may help to improve the early diagnosis of acute coronary syndromes in patients presenting to the Emergency Department (ED) with chest pain. A novel qualitative point of care h-FABP lateral flow immunoassay (True Rapid, FABPulous BV) could provide results to clinicians within just 5 minutes. Given the qualitative nature of this test and prior to evaluation in a large diagnostic study, we aimed to determine inter-observer reliability when interpreted contemporaneously by staff in the ED.<\/p>\n

Methods<\/em><\/strong><\/p>\n

In a nested prospective cohort study including adult patients with suspected cardiac chest pain, venous blood samples were tested for h-FABP (FABPulous BV) on arrival and 3 hours later. Each test result was independently interpreted by two different investigators after 5 minutes. The investigators were blinded to each other\u2019s interpretation and recorded their findings on separate case report forms. We determined interobserver reliability by calculating the Cohen\u2019s kappa score and 95% confidence intervals.<\/p>\n

Results<\/em><\/strong><\/p>\n

A total of 43 test results (from 31 patients) were each interpreted by two independent investigators. Absolute agreement between investigators was 93.0%, with a Cohen\u2019s kappa of 0.81 (95% CI 0.6 \u2013 1.0), indicating near perfect agreement.\u00a0 In total there were three (7.0%) disagreements.\u00a0 In each case one investigator reported a \u2018weak positive\u2019 result while the other interpreted the result as \u2018negative\u2019.<\/p>\n

Conclusions<\/em><\/strong><\/p>\n

These findings demonstrate the interobserver reliability of a qualitative point of care h-FABP assay. Further work must evaluate diagnostic accuracy and determine the clinical implications of the small rate of disagreement.<\/p>\n\nLink to Journal<\/span><\/a>","protected":false},"excerpt":{"rendered":"

Mashali, M., Norris, N., McDowell, G. & Body, R. (2016) The inter-observer reliability of a novel qualitative point of care assay for heart-type fatty acid binding protein. Clin Biochem 49 pp. 1199-1201 \u00a0 Background Heart-type fatty acid\u2013binding protein (h-FABP) may<\/p>\n","protected":false},"author":1,"featured_media":153,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"_links_to":"","_links_to_target":""},"jetpack_shortlink":"https:\/\/wp.me\/P9tPlw-4b","jetpack-related-posts":[{"id":641,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=641","url_meta":{"origin":259,"position":0},"title":"A FABP-ulous rule out strategy","date":"February 7, 2019","format":false,"excerpt":"Body, R.,\u00a0McDowell, G., Carley, S., Wibberley, C., Ferguson, J. & Mackway-Jones, K. (2011) \u2018A FABP-ulous rule out strategy? Heart fatty acid binding protein and troponin for rapid exclusion of acute myocardial infarction.\u2019\u00a0Resuscitation82 pp. 1041-1046.\u00a0 Objective:Many Emergency Departments (EDs) utilise \u2018triple marker\u2019 testing with CK-MB, myoglobin and troponin I (cTnI) to\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"https:\/\/i0.wp.com\/clinicalbiochemistry.net\/wp-content\/uploads\/2019\/01\/Quanti-HFABP-e1549807872944.png?fit=400%2C341&resize=350%2C200","width":350,"height":200},"classes":[]},{"id":549,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=549","url_meta":{"origin":259,"position":1},"title":"PhD Project: BEST Study","date":"January 11, 2019","format":false,"excerpt":"Expediting the confirmation of acute myocardial infarction with point of care troponin and heart fatty acid binding protein testing to facilitate early intervention in emergency department Cardiac troponin is the reference standard biomarker for the diagnosis of acute myocardial infarction (AMI). In the appropriate clinical context, the detection of a\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":121,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=121","url_meta":{"origin":259,"position":2},"title":"Research Papers","date":"December 22, 2017","format":false,"excerpt":"Body, R., McDowell, G., Carley, S., Ferguson, J. & Mackway-Jones, K. (2010) \u2018Diagnosing acute myocardial infarction with troponins: how low can you go?\u2019 Emerg Med J. 27 pp. 292-296. Body, R., Carley, S., Wibberley, C., McDowell, G., Ferguson, J. & Mackway- Jones, K. 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Emerg Med J 34 pp. 349-356 Background The original Manchester\u2026","rel":"","context":"Similar post","img":{"alt_text":"","src":"https:\/\/i0.wp.com\/clinicalbiochemistry.net\/wp-content\/uploads\/2017\/12\/Cardiac-Marker-Test-Pages-300x200.png?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":659,"url":"http:\/\/clinicalbiochemistry.net\/?page_id=659","url_meta":{"origin":259,"position":4},"title":"MACS Rule","date":"February 7, 2019","format":false,"excerpt":"Body, R., Carley, S.,\u00a0McDowell, G., Pemberton, P., Burrows, G., Cook, G., Lewis, P.S., Smith, A., Mackway-Jones, K. 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(2018) Acetylcholine inhibits monomeric C-Reactive Protein inducd inflammation, endothelial cell adhesion and platelet aggregation; A potential therapeutic. 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